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Rotating the internal valve (proven in red) to the inject position directs the mobile period with the sample loop and on to the column.
物質の濃度により光の通過する角度が変わることを利用した検出器。原理上グラジェント分析はできない（グラジェントによって移動相自体の屈折率が変化するため）。また、感度が低いのが欠点だが、大部分の物質に対して使用できる。
The simplest way to value the theoretical and the practical information talked about In this particular portion is always to cautiously look at a typical analytical method.
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シリカゲルの粒子径が小さければ小さいほどピークの分離性は良くなるが、送液に必要なポンプの圧力が高くなる。そのため、ポンプ－インジェクター間、インジェクター－カラム間の配管の耐圧を上げたり、カラム自体を比較的高温の下にさらして溶媒の粘度を下げ、抵抗を小さくする工夫をしている。
As the cellular stage flows through the column, the compounds during the sample interact with the stationary phase. This interaction will cause the compounds to separate based on their precise Attributes, like polarity, size, charge, or affinity.
Acid–base chemistry is not the only example of a secondary equilibrium response. Other illustrations consist of ion-pairing, complexation, as well as interaction of solutes with micelles. We'll consider the last of these in Chapter twelve.7 whenever we explore micellar electrokinetic capillary chromatography.
Ghost peaks are extraneous peaks that look during the chromatogram but Never correspond to any parts during the sample. These can complicate details Evaluation. Below are a few prospective brings about and options:
Broadened peaks can obscure goal peaks and make quantification hard. Here are several frequent triggers and methods for here peak broadening:
- 분석물의 분리여부는 고정상(컬럼)과 이동상의 조합에 의해 결정합니다.(실제 시료 측정에서는 시료 중에 분석물 이외의 오염물질에 존재하는 경우가 많아 분석자는 그 시료의 측정에 최적인 분석 조건의 검토가 필요합니다.
A lot of differing kinds of detectors are actually use to watch HPLC separations, the majority of which utilize the spectroscopic strategies from Chapter ten or perhaps the electrochemical methods from Chapter eleven.
Sample carryover: Sample parts can continue to be within the system just after an injection, leading to them to look in subsequent injections as ghost peaks. Make sure good rinsing on the injection system between injections. Take into account raising the wash volume or utilizing a more robust get more info clean solvent.
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